Pillar 1: Ubiquitome Discovery & Degron Logic

Mapping protein fate, PTM-dependent degrons, and context-specific routing. Ubiquitome-wide discovery engine – substrate repertoire, modulation by compounds with comparative mass spectrometry. Compound screening with precision and throughput.

Pillar 2: Mechanistic Assay Systems

Cell-based assay for compound-specific engagement mapping, cooperativity measurement, and surrogate degron detection. Pathway analysis, consequences of drug-action. Utilizes recombinant expression of proprietary peptide each targeting proteins of interest in model cell systems.

Pillar 3: Small Molecule Mechanistic Mapping

Catalytic inhibition, SUMO-switch logic, and JAK-STAT crosstalk. Combination of UBQuest, PINTAC and SMARTRaP assays – complimentary data show mechanistics and ternary complex formation.

Pillar 4: Peptide Discovery & Cohort Engineering

Motif discovery, cohort reconstruction, and proteostasis restoration. Products of computational analysis of large amounts of proteomic data from our platforms.

Pillar 5: Glue & Ternary Complex Engineering

TSA-CRBN assay, BRD4 kinase screening, and therapeutic peptides in Parkinson’s disease and Alzheimers.

UBQuest – Whole-Ubiquitome Cell-Based Screening

UBQuest screens compounds across the entire ubiquitome in live cells, enabling global coverage of all E3 ligases, DUBs, and proteasomal recruiters. This provides unparalleled breadth for discovering degradation events and ubiquitin-dependent mechanisms that targeted assays cannot capture.

PINTAC – Target-Specific Biology, PTMs, and Pathways

PINTAC uses proprietary peptides to interrogate specific proteins of interest, mapping post-translational modifications, pathway-level interactions, and context-specific substrate engagement. This platform delivers high-resolution biological clarity that complements UBQuest’s global scan.

SMARTRaP – Solid-Phase Ternary Complex and RIPTAC Screening

SMARTRaP is a high-throughput solid-phase assay designed to resolve ternary complex formation, RIPTAC binding proteins, and small-molecule engagement kinetics. It provides mechanistic validation at scale, confirming the assemblies that drive targeted protein degradation.

Unmatched Sensitivity and Mechanistic Clarity

Across all platforms, VistaraBio achieves:

• 50-fold enrichment
• Identification of over 40,000 peptides via mass spectrometry
• Sensitivity approaches 1 in 3,000,000 stoichiometry of bioactive regions in clinically important proteins.
• Highly reproducible in replicates and compounds of similar function – e.g., BET inhibitor class.

This sensitivity and precision of detection is unprecedented in the industry and enables:

• Mechanistic clarity including mechanism of action (MoA)
• Accurate efficacy prediction
• Safety and off-target resolution
• Context-specific substrate mapping

Together, UBQuest, PINTAC, and SMARTRaP form a globally comprehensive and mechanistically precise operating system for molecular glue and RIPTAC discovery.